ARDEP 1005

Development of a Smartphone-Integrated Reader System Using the Horizontal Flow Immunoassay Method for Rapid, Quantitative Detection of Alpha-1-Acid Glycoprotein (AGP) in Cats

The presented project proposal includes an indigenous lateral flow test kit and quantitative analysis of these kits, which will determine the concentration of Alpha-1-acid glycoprotein (AGP), an important AFP, in healthy and sick individuals in order to monitor and determine the severity of infection in cats. It is about the development of reader systems that will provide For this purpose, with the project in question, a model product will be introduced to reduce our foreign dependency in the field of veterinary clinical services and to increase the country's competitiveness in this field. With the reader systems to be developed, the production of kits that provide rapid diagnosis of important diseases and biomarkers in the field of Veterinary Internal Medicine will be planned and development studies will continue. Therefore, the validated LFA prototype and reader mobile system obtained from this project will be a pioneering step in the development of projects that will identify other analytes. Thanks to the prototype to be produced, the validity, sensitivity and specificity of AGP analyzes in cat serum under laboratory and field conditions will be tested. After all the tests, the validated kit will be patented. The kit, which is planned to be produced in the field of veterinary AFP, may become a product that will be demanded by veterinary small animal clinics all over the world, as it is a new analytical biomarker that will be rapidly developed with LFT. Therefore, Türkiye will have a product that can compete with other AFP kits in the field of veterinary medicine. In addition, thanks to the development of rapid diagnostic kits that will enable the diagnosis of biochemical analytes, it will provide a national gain as a product that will increase the country's competitiveness in the veterinary diagnostic sector. This practical kit and mobile reader system, which will determine the concentration of AGP in body fluids such as serum, plasma or peritoneal fluid, is a diagnostic tool that will enable the monitoring of inflammation and infection processes, which has no equivalent in small animal medicine, and will be presented on a product basis for the first time. In this way, it will be a unique product that will gain national patents. Among such products, which are under continuous development, they have found application in veterinary medicine and are currently used to determine different analytes such as serum Amyloid A (SAA) and C-Reactive Protein (CRP). Practical rapid diagnostic kits and reader systems, either integrated into smart mobile phones or as a separate device, that can be at least as effective in the field as these biomarkers, do not require practical and lengthy laboratory procedures, and determine AGP levels at a lower cost, are not yet available in our field. For this reason, the project proposal presented will enable the introduction of a new and original diagnostic tool in the field of veterinary clinical diagnosis, not only in our country but all over the world.

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